Exonuclease III, 200 U/µL, 25,000 UDigest duplex DNA in a 3 to 5 direction from a nick, blunt end, or 3 recessed end, producing stretches of ssDNA. Key features Produce stretches of ssDNA in dsDNA templates containing nicks, blunt ends or recessed 3' ends Protect protruding 3' ends because this exonuclease will not start digestion at protruding 3' overhangs Product information Exonuclease III digests duplex DNA in a 3 to ; 5 direction from a nick, a blunt end, or 3 recessed end,