Trypsin 1:250Trypsin 1: 250 is used in the preparation of trypsin EDTA solution, which is commonly utilized for detaching adherent cells from the culture vessel for subculturing or passaging. Please see some applications below: Cell detachment: Trypsin 1: 250 is an enzyme that breaks down proteins, including the proteins that anchor cells to the culture vessel. When cells reach confluence or need to be passaged, trypsin EDTA solution is added to the cell culture