AmpFi RT MasterMixAmpFi RT MasterMix (DM AmpRT ) ensures accurate qPCR results where contaminating gDNA would otherwise result in artificially early Ct values. RNA samples contaminated with gDNA were reverse transcribed for 25 min with AmpFi RT MasterMix . 1I of RT product was then used in qPCR to assay amplification of a GAPDH target. Store at 20C. AmpFi RT MasterMix was designed to perform simple, reliable, and reproducible first strand cDNA synthesis. Reagent